Anesthetic Profile of a Non-lipid Propofol Nanoemulsion

SummaryBackground and objectivesThe clinical use of a lipid propofol formulation causes pain during injection, allergic reactions, and bacterial growth. Propofol has been reformulated in different non-lipid presentations to reduce the incidence of adverse effects, but those changes can modify its pharmacokinetics and pharmacodynamics. In the present study, we investigate the pharmacology and toxicology of lipid propofol (CLP) and the non-lipid nanoemulsion (NLP).MethodsConventional lipid formulation of propofol and NLP were infused in the jugular veins of rats and blood pressure (BP), heart rate (HR), and respiratory rate (RR) were measured. Both formulations (1%) were infused (40 μL.min-1) over 1 hour. Hypnotic and anesthetic doses as well as recoveries were determined. The pain induced by the CLP and NLP vehicles was compared by counting the number of abdominal contortions (“writhing test”) after the intraperitoneal (i.p.) injection in mice. Acetic acid (0.6%) was used as positive control.ResultsHypnotic and anesthetic doses of 1% CLP (6.0 ± 1.3 and 17.8 ± 2.6 mg.kg-1, respectively) and 1% NLP (5.4 ± 1.0 and 16.0 ± 1.4 mg.kg-1, respectively) were not significantly different. Recovery from hypnosis and anesthesia was faster with NLP than with CLP. Changes in HR, BP, and RR caused by NLP were not significantly different from those caused by CLP. Acetic acid and the vehicle of CLP caused 46.0 ± 2.0 and 12.5 ± 0.6 abdominal contortions 20 min after i.p. injection, respectively. The absence of abdominal contractions was observed with the vehicle of NLP. Abdominal inflammatory response was not observed after the i.p. injection of both propofol vehicles.ConclusionsNon-lipid formulation of propofol can be a better alternative to CPL for intravenous anesthesia with fewer adverse effects

A New Laparoscopic Surgical Procedure to Achieve Sufficient Mesorectal Excision in Upper Rectal Cancer

Objective. Mesorectal excision corresponding to the location of a tumor, termed tumor-specific mesorectal excision (TSME), is commonly performed for resection of upper rectal cancer. We devised a new laparoscopic procedure for sufficient TSME with rectal transection followed by mesorectal excision. Operative Technique. After mobilization of the sigmoid colon and ligation of inferior mesenteric vessels, we dissected the mesorectum along the layer of the planned total mesorectal excision. The rectal wall was carefully separated from the mesorectum at the appropriate anal side from the tumor. After the rectum was isolated and transected using an endoscopic linear stapler, the rectal stump drew immediately toward the anal side, enabling the mesorectum to be identified clearly. In this way, sufficient TSME can be performed easily and accurately. This technique has been successfully conducted on 19 patients. Conclusion. This laparoscopic technique is a feasible and reliable procedure for achieving sufficient TSME

Ka-Band Multi-Gbps High Speed Downlink With Electrically Steerable Beam

With advances in sensor technology and data services, the latest earth observation missions tend to require substantial amounts of remote sensing data to be downlinked to earth stations with shorter delivery times. To meet these demands, AXELSPACE is developing a Ka-band downlink system (AxelLink-Ka) in cooperation with Tokyo Institute of Technology 1 that combines a broadband Ka-band transmitter and an active phased array antenna.2 The prototype of the AxelLink-Ka was found to achieve a data rate of up to 4.2 Gbps with using dual polarizaion of Right- and Left-Handed Circular Polarization (RHCP/LHCP) in the Ka-band (25.5—27.0 GHz) and electrical beam steering covering ±66-degree range with the small enough Size, Weight and Power (SWaP) to be installed on a microsatellite: the size ∼ 220 × 170 × 100 mm, the weight \u3c 2, 300g, and the power consumption \u3c 60 W. This paper provides an overview of the entire system, simulation results of link budgets, and design and measurement results of the hardware and software

Lokiarchaeota archaeon schizorhodopsin-2 (LaSzR2) is an inward proton pump displaying a characteristic feature of acid-induced spectral blue-shift

The photoreactive protein rhodopsin is widespread in microorganisms and has a variety of photobiological functions. Recently, a novel phylogenetically distinctive group named 'schizorhodopsin (SzR)' has been identified as an inward proton pump. We performed functional and spectroscopic studies on an uncharacterised schizorhodopsin from the phylum Lokiarchaeota archaeon. The protein, LaSzR2, having an all-trans-retinal chromophore, showed inward proton pump activity with an absorption maximum at 549 nm. The pH titration experiments revealed that the protonated Schiff base of the retinal chromophore (Lys188, pK(a)=12.3) is stabilised by the deprotonated counterion (presumably Asp184, pK(a)=3.7). The flash-photolysis experiments revealed the presence of two photointermediates, K and M. A proton was released and uptaken from bulk solution upon the formation and decay of the M intermediate. During the M-decay, the Schiff base was reprotonated by the proton from a proton donating residue (presumably Asp172). These properties were compared with other inward (SzRs and xenorhodopsins, XeRs) and outward proton pumps. Notably, LaSzR2 showed acid-induced spectral 'blue-shift' due to the protonation of the counterion, whereas outward proton pumps showed opposite shifts (red-shifts). Thus, we can distinguish between inward and outward proton pumps by the direction of the acid-induced spectral shift

On-line Overlaid-Handwriting Recognition Based on Substroke HMMs.

This study discusses the subject of training data selection for neural networks using back propagation. We have made only one assumption that there are no overlapping of training data belonging to different classes, in other words the training data is linearly/semi-linearly separable . Training data is analyzed and the data that affect the learning process are selected based on the idea of Critical points. The proposed method is applied to a classification problem where the task is to recognize the characters A,C and B,D. The experimental results show that in case of batch mode the proposed method takes almost 1/7 of real and 1/10 of user training time required for conventional method. On the other hand in case of online mode the proposed method takes 1/3 of training epochs, 1/9 of real and 1/20 of user and 1/3 system time required for the conventional method. The classification rate of training and testing data are the same as it is with the conventional method

Pen pressure features for writer-independent on-line handwriting recognition based on substroke HMM

This paper discusses the use of pen pressure as a feature in writer-independent on-line handwriting recognition. We propose two kinds of features related to pen pressure: one is the pressure representing pen ups and downs in a continuous manner; the other is the time-derivative of the pressure representing the temporal pattern of the pen pressure. Combining either of them with the existing feature (velocity vector), a 3-dimensional feature is composed for character recognition. Some techniques of interpolating the pen pressure during the pen-up interval is also proposed for a pre-processing purpose. Through experimental evaluation using 1,016 elementary Kanji characters compared with the baseline performance using velocity vector only, the additional use of pen pressure improved the performance from 97.5% to 98.1% for careful writings and from 91.1% to 93.1% for cursive writings

Human Hematopoietic Stem Cells Can Survive In Vitro for Several Months

We previously reported that long-lasting in vitro hematopoiesis could be achieved using the cells differentiated from primate embryonic stem (ES) cells. Thus, we speculated that hematopoietic stem cells differentiated from ES cells could sustain long-lasting in vitro hematopoiesis. To test this hypothesis, we investigated whether human hematopoietic stem cells could similarly sustain long-lasting in vitro hematopoiesis in the same culture system. Although the results varied between experiments, presumably due to differences in the quality of each hematopoietic stem cell sample, long-lasting in vitro hematopoiesis was observed to last up to nine months. Furthermore, an in vivo analysis in which cultured cells were transplanted into immunodeficient mice indicated that even after several months of culture, hematopoietic stem cells were still present in the cultured cells. To the best of our knowledge, this is the first report to show that human hematopoietic stem cells can survive in vitro for several months

Further studies on an eleventh case of heavy (Hgamma1) chain disease--clinical studies

An eleventh case of heavy (Hgamma1) chain disease (Yok), surviving for more than 10 years and still living showed clinical and pathological findings similar to cases described in the past. The patient was given only glucocorticosteroids, ACTH, antibiotics and gamma globulin, as specific drugs. Precipitation arcs besides the major ones formed by albumin and Fc fragment were disclosed by immunoelectrophoresis. The existence of these minor components were confirmed with antigen-antibody crossed electrophoresis and Sephadex G-200 gel filtration. They did not form precipitation arcs with the other antigens available and they appeared in the same fractions of IgG on gel filtration suggesting their having higher molecular weight than the major ones. In addition to these findings, the clinical course of the patient is described.</p